Figure 3
![Figure 3. The rate of inactivation of FXIII-A2* can occur on a physiologically relevant timescale. (A) Lineweaver-Burk plot used to generate kinetic parameters for FXIII-A2*, with data sets represented by different colors. The data point circled in pink is derived from the slope of the initial velocity in panel B (pink line). (B) The generation of FXIII-A2* cleavage products by plasmin (300 nM) at a representative FXIII-A2* concentration, determined using an ammonia release assay. (C) Standard curves of ammonia generation over time using various [FXIII-A2*]. (D) Ammonia generation over time, with varying times of FXIII-A2* digestion with plasmin. n = 3 for all experiments. Abs, absorbance.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/126/20/10.1182_blood-2015-07-650713/4/2329f3.jpeg?Expires=1724936337&Signature=yX8nrAkboE9BuN2Be6XkpobUggznqvZjoEyfQP007A3NH~0fzb0ekX7nF4cYAHEDD8yXWh-fmDg9JVwgg1zPGu02vAxeX5RKPZA9L~c80mB90fEMD2iVyyzG1yrEkc7d1mNAxkte1VOhYzXRohJtIxoUntYJ7Wl3Zrkv~Ktaj993EWwY9aiumFxijTzLzOg71PApaugJB9HUjObw0~QkeG1k-apJTcVsSt-CxJoAZjEcYFtyLkear6M5BMuwSdg8qkxcOD~kZqt~WZ2oAjeNiV9OlaX2zyxmaHexYm9DzzmPDpqPeN15SUo~BcehMwykPPD2W6Gwz9XTyeC2ZFfUow__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The rate of inactivation of FXIII-A2* can occur on a physiologically relevant timescale. (A) Lineweaver-Burk plot used to generate kinetic parameters for FXIII-A2*, with data sets represented by different colors. The data point circled in pink is derived from the slope of the initial velocity in panel B (pink line). (B) The generation of FXIII-A2* cleavage products by plasmin (300 nM) at a representative FXIII-A2* concentration, determined using an ammonia release assay. (C) Standard curves of ammonia generation over time using various [FXIII-A2*]. (D) Ammonia generation over time, with varying times of FXIII-A2* digestion with plasmin. n = 3 for all experiments. Abs, absorbance.
