Microarray-based gene expression profiling of primary B cells and B-cell lymphomas. Heat map of RNA gene expression profiles from splenic B cells (ATMWT [WT] and ATMKO [KO]), treated with or without goat-anti-mouse IgM (15 μg/mL) stimulation (at 0, 3, 6, and 12 hours), ATMWT GC B cells (GCB), and 9 ATMKO.CD3εKO tumors. RNA was labeled with Cy5 and compared to a common pool of reference RNA labeled with Cy3 to allow direct comparison of all samples to each other. Clusters of genes with similar behaviors were identified (supplemental Table 1) and captured for subsequent signature analysis (supplemental Table 2) to identify enriched functions and pathways. Genes representative of a particular function are listed on the right. Relative differences in expression are indicated by the color bar; * indicates a technical replicate of that sample.