RBC, hemoglobin, Hamp mRNA, and Erfe mRNA levels after transfusion in thalassemic mice. RBC (A) and hemoglobin (B) were significantly increased in Th3/+ mice 48 hours after transfusion of 400 µL packed red cells. Hamp mRNA expression was increased in the liver, whereas Erfe mRNA levels were decreased and returned to baseline in the bone marrow and spleen of transfused Th3/+ mice compared with saline-treated control mice. (C-D) Data shown are means ± SEM and were compared between control and transfused Th3/+ mice (n = 3 per group) by 2-tailed Student t-test. **P < .01.