Knockout and overexpression of miR-126 promote AE9a-induced leukemogenesis in primary and secondary BMT. (A) The effect of the overexpression and knockout of miR-126 on AE9a-induced primary leukemogenesis in primary BMT recipient mice. Kaplan-Meier curves and P values (log-rank test) are shown. (B) Expression of miR-126 was detected by qRT-PCR assay in leukemic or control BM cells (CD45.2⁺) isolated from primary BMT recipient mice at the end point. **P < .01. Survival curves (C) and relative miR-126 expression levels (D) in secondary BMT recipient mice are shown. **P < .01. (E) Wright-Giemsa–stained PB and BM, and H&E-stained spleen and liver of the secondary BMT recipient mice at the end point. Bars represent 25 μm for PB and BM; 100 μm for spleen and liver.