GILZ regulates B-cell homeostasis in BM. (A) Number of cells in BM of 8- to 12-week-old WT and gilz KO mice. (B) Flow cytometry analysis of B220 expression (left) in cells derived from BM of 8- to 12-week-old WT and gilz KO mice. On the right are the frequency and number of B220⁺ cells in BM isolated from 8- to 12-week-old WT and gilz KO mice. (C) Flow cytometry analysis of B220 and CD43 expression in B220⁺/lo cells of BM of 8- to 12-week-old WT and gilz KO mice. (D) Flow cytometry analysis of cKIT expression in B220loCD43⁺ cells of BM of 8- to 12-week-old WT and gilz KO mice. (E) Frequency and number of PreProB (B220loCD43⁺cKIT^–), or ProB (B220loCD43⁺cKIT⁺) (F). (G) Flow cytometry analysis of IgM and IgD expression in B220⁺CD43^– cells of BM of 8- to 12-week-old WT and gilz KO mice. (H-J) Frequency and number of PreB cells (B220⁺CD43^–IgM^–IgD^–) (H), immature B cells (B220⁺CD43^–IgM⁺IgD^–) (I), or mature B cells (B220⁺CD43^–IgM^−/+IgD⁺) (J) isolated from 8- to 12-week-old WT and gilz KO mice. Each symbol represents an individual mouse; small horizontal lines indicate the mean. Data were derived from 7 independent experiments. *P < .05, **P < .005, ***P < .0005.