CK2 inhibition and Ikaros overexpression similarly affect transcription of genes that control cell cycle progression and the PI3K pathway in vitro and in vivo. (A-B) Heatmap of qRT-PCR analysis of Ikaros target genes that promote (A) cell cycle and (B) the PI3K pathway. Expression in controls: wild-type Nalm6, Nalm6 transduced with empty vector, and Nalm6 transduced with scramble shRNA (lanes 1-3); in Nalm6 with overexpressed Ikaros (lane 4); in Nalm6 treated with CK2 shRNA (lane 5); and Nalm6 following CK2 inhibition, in vitro (with TBB) (lanes 6-7), or in vivo (with CX-4945) (lanes 8-9). (C) Ikaros knockdown partially rescues transcriptional repression following CX-4945 treatment of Nalm6 cells in vivo. Nalm6 cells stably transfected with Ikaros shRNA or scramble shRNA (control) and selected with puromycin were injected into NSG mice and treated with CX-4945 for 3 days. Gene expression was measured by qRT-PCR in cells harvested from engrafted leukemia. (D) Western blots of Nalm6 xenografts showing that CK2 inhibition decreases phosphorylation of AKT (pAKT) (upper), whereas levels of AKT are unchanged (lower). The relative intensity of bands by densitometry scanning are shown on the right.