Genetic loss of Tr2/Tr4 abolishes the chromatin occupancy of TR2/TR4 and their cofactors at the DR-binding sites in the embryonic globin gene promoters. The binding of TR2/TR4 and other corepressors to select murine β-like globin gene promoters: εy (A), βh1 (B), and β^maj (C) as well as to an irrelevant control sequence located 5.9 kbp 5′ to the β^maj promoter (D) in infected Lin⁻ cells were analyzed in ChIP experiments. Statistically significant enrichment of the orphan receptors and/or the cofactors at the promoters and control sequence is indicated in comparison with the control IgG values (indicated with asterisks; *P < .05).