Foxc2 is a downstream mediator of Notch signaling in AGM hematopoiesis. (A) Low-dose foxc1a/b morpholinos (4/4 ng) were injected into fertilized zebrafish eggs from a wild-type cross or from a cross of hsp70:gal4 and uas:NICD. Heat shock was performed on embryos from hsp70:gal4 and uas:NICD cross between 8 and 12 somite stages at 37°C for 30 minutes to induce NICD expression. Embryos were incubated at 28°C. Wild-type crosses were harvested at 28 hpf and 36 hpf, and hsp70:gal4 and uas:NICD crosses were harvested at 36 hpf. In situ hybridization with flk1 (wild-type at 28 hpf) or c-myb (wild-type and NICD at 36 hpf) is shown. Arrowhead indicates positive c-myb staining. Morpholino injection decreased (7 partial + 23 complete/30) c-myb expression, and NICD induction did not rescue the decrease (1 partial + 6 complete/7). (B) Jagged1 null mouse embryos and littermates (E10.5) were subjected to in situ hybridization with Foxc2 probe. Transverse sections of dorsal aorta in the AGM region are shown in a dorsal to ventral orientation. Two representative Jagged1 null embryos are shown (n = 4).