Foxc2 expression is upregulated in VE SP population after ICN1 induction. (A) CD45⁻ CD41 SP, VE SP, DP, and double negative (DN) populations were sorted from day 6 iICN1 EBs with or without Dox induction (0.5 μg/mL) from days 3 to 5 during EB differentiation and subjected to microarray analysis for gene expression. Hierarchical clustering of populations is shown (Euclidean metric). The numbers, which are applied afterward, represent the corresponding sorted populations. (B) Genes with a similar expression pattern to Gata3 are indicated (Spearmann metric with cutoff 0.95 followed by Pearson metric with cutoff 0.9). Expression levels relative to average gene expression are plotted. (C) Genes with an increase in expression by twofold or higher after ICN1 induction in CD41 SP, VE SP, and DP population are listed from high to low expression. Genes showing an expression pattern similar to Gata3 are marked by a rectangular shade. Data were collected from 3 independent experiments.