The anticoagulant effects of long-chain ACs in different clotting assays were determined. Effects of various doses of 16:0-AC are shown for clotting assays in which clotting was induced by endogenously generated factor Xa (RVV-X activated), exogenously added factor Xa, diluted TF, or thrombin. (B) Effect of length of aliphatic side chain of ACs (6:0, 8:0, 10:0, 14:0. 16:0, 18:0, and 18:2) on their anticoagulant activity, measured using RVV-X clotting time.