Ly6C expression marks a subset of mouse bone marrow GMPs. (A) Ly6C expression by LKS⁻CD34⁺FcγR^hi cells (unsorted GMPs) was assessed by flow cytometry (see “Materials and methods” and supplemental Figure 1 for sorting and gating strategies). Data are representative of >3 independent experiments. (B-C) GMP-Ly6C⁻ and GMP-Ly6C⁺ cells were FACS sorted from CD45.2 donor mouse Lin⁻ cells and 200 000 cells were injected IV into congenic CD45.1 recipient mice (nonirradiated) on day 0. Spleens were harvested from recipient mice on days 4 and 7, and donor cell-derived (CD45.2⁺) neutrophils (green gates), monocytes, and macrophages (blue gates) were detected by flow cytometry (see supplemental Figure 4 for gating strategy). CD45.2⁺CD11b⁺ splenic cells are shown. Data presented are from 1 experiment that is representative of at least 3 independent experiments.