Patient neutrophils display phagocytosis and migration defects. (A) Family tree. (B-C) Fluorescein isothiocyanate (FITC) –positive Escherichia coli uptake by neutrophils analyzed by flow cytometry. Representative data from 2 independent experiments. (D) Migration of control and patient neutrophils in a Dunn chamber in response to an fMLP gradient. Tracks show migration path over time. The number of cells analyzed was 41 in the control and 110 and patient sample in 1 experiment. Scale bar = 10 μm. (E) Frequency distribution of the velocity of patient and control neutrophils. (F) Chemotactic index of the migrating neutrophils. (G) Distribution of instantaneous angle between the gradient and the cell path during migration. (H) Frequency distribution of the velocity of control and patient B lymphoblastoid cell lines migrating in collagen gel. Twenty cells were analyzed in each population in 1 experiment. Data are representative of 2 independent experiments. *** P < .001. MFI, mean fluorescent intensity.