Megakaryocyte- and platelet-specific Atg7 deletion causes impaired hemostasis and thrombus formation without thrombocytopenia. (A) Tail-bleeding time measurements taken from Atg7^f/f (○) and Atg7^f/f;PF4-Cre/+ (filled gray triangles) mice. (B) Occlusion time measurements taken from Atg7^f/f (○) and Atg7^f/f;PF4-Cre/+ (filled gray triangles) mice in the FeCl₃ carotid injury model of thrombosis. For A and B, each data point represents an individual mouse, with the horizontal line marking the median. Statistical significance was evaluated with the log-rank test using SigmaPlot12 software. (C) Platelet counts and (D) mean platelet volumes from Atg7^f/f (○) and Atg7^f/f;PF4-Cre/+ (filled gray triangles) mice. For C and D, statistical significance was evaluated with the Student t test. NS, not significant.