Figure 1
Figure 1. Treatment with the BET antagonists JQ1 and I-BET151 induces cell-cycle growth arrest and lethal effects in cultured MCL cells. (A) Cell-cycle status of MO2058 (left) and Mino (right) cells following 24 hours of treatment with JQ1, as indicated. Columns, mean of 3 independent experiments; Bars, ± standard error of the mean (SEM). (B) MO2058 and Mino cells were cultured in the presence or absence of 1.0 µM of JQ1 and cell counts were measured every 24 hours for 120 hours. Lines represent the mean cell number from 3 experiments ± standard deviation (SD). (C) MO2058, JeKo-1, Mino, and Z-138 cells were treated with the indicated concentrations of JQ1 for 48 hours. The percent of Annexin V-positive apoptotic cells was determined by flow cytometry. Columns, mean of 3 independent experiments; Bars, ± SEM. (D) MO2058 and JeKo-1 cells were treated with the indicated concentrations of I-BET151 for 48 hours. Annexin V-positive apoptotic cells were determined by flow cytometry. Columns, mean of 3 independent experiments; Bars, ± SEM. IC50 values were calculated using GraphPad Prism software (version 5). (E) Primary MCL cells were treated with the indicated concentrations of JQ1 for 48 hours. The percent of nonviable cells was determined by flow cytometry. Columns, mean percent loss of viability of 6 primary MCL samples; Bars, ± SEM. (F) MO2058 (left) and Mino (right) cells were cocultured with or without HS5 stromal cells and then treated with JQ1, as indicated, for 48 hours. The percent of apoptosis of the MO2058 and Mino or HS5 cells was determined by staining with Annexin V and TO-PRO-3 iodide and flow cytometry. Columns represent the mean apoptosis of 3 independent experiments; Bars, ± SEM. (G) Primary MCL cells were cocultured with or without HK stromal cells and then treated with JQ1 for 48 hours. The percent of nonviable cells was determined by PI staining and flow cytometry. Asterisk (*) indicates loss of viability values significantly less (P < .05) in cells cocultured with HK stromal cells compared with those without coculture.

Treatment with the BET antagonists JQ1 and I-BET151 induces cell-cycle growth arrest and lethal effects in cultured MCL cells. (A) Cell-cycle status of MO2058 (left) and Mino (right) cells following 24 hours of treatment with JQ1, as indicated. Columns, mean of 3 independent experiments; Bars, ± standard error of the mean (SEM). (B) MO2058 and Mino cells were cultured in the presence or absence of 1.0 µM of JQ1 and cell counts were measured every 24 hours for 120 hours. Lines represent the mean cell number from 3 experiments ± standard deviation (SD). (C) MO2058, JeKo-1, Mino, and Z-138 cells were treated with the indicated concentrations of JQ1 for 48 hours. The percent of Annexin V-positive apoptotic cells was determined by flow cytometry. Columns, mean of 3 independent experiments; Bars, ± SEM. (D) MO2058 and JeKo-1 cells were treated with the indicated concentrations of I-BET151 for 48 hours. Annexin V-positive apoptotic cells were determined by flow cytometry. Columns, mean of 3 independent experiments; Bars, ± SEM. IC50 values were calculated using GraphPad Prism software (version 5). (E) Primary MCL cells were treated with the indicated concentrations of JQ1 for 48 hours. The percent of nonviable cells was determined by flow cytometry. Columns, mean percent loss of viability of 6 primary MCL samples; Bars, ± SEM. (F) MO2058 (left) and Mino (right) cells were cocultured with or without HS5 stromal cells and then treated with JQ1, as indicated, for 48 hours. The percent of apoptosis of the MO2058 and Mino or HS5 cells was determined by staining with Annexin V and TO-PRO-3 iodide and flow cytometry. Columns represent the mean apoptosis of 3 independent experiments; Bars, ± SEM. (G) Primary MCL cells were cocultured with or without HK stromal cells and then treated with JQ1 for 48 hours. The percent of nonviable cells was determined by PI staining and flow cytometry. Asterisk (*) indicates loss of viability values significantly less (P < .05) in cells cocultured with HK stromal cells compared with those without coculture.

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