Strongly decreased FRAP in proplatelets treated with cytoplasmic dynein inhibitors. (A) Representative images of time-lapse microscopy after photobleaching of ROIs (dashed arrows). White arrow in zoomed area indicates microtubule entering photobleached ROI. Indicated numbers represent seconds. pre, before photobleaching. Scale bar is 10 µm. (B-F) Cells were preincubated with indicated agonists, and fluorescence intensity in photobleached ROI was measured. n = at least 10 per condition. P values after photobleaching: nocodazole treatment to control: before t = 255 seconds (not significant), and after t = 255 seconds (not significant, **P < .01, *P < .05); EHNA treatment to control (***P < .001, **P < .05), and Ciliobrevin D and taxol treatment to control (***P < .001 for all time points).