Inflammasome activity evident in recovered MDSCs. (A) Western blot of cell lysates from recovered wild-type or ASC^−/− MDSC-IL13 probed for the active p10 form of caspase-1 and β-actin. ImageJ software was used to convert to grayscale and straighten and crop the gel image to highlight lanes of interest according to size. (B) Caspase-1 p10 blot quantification relative to β-actin; GVHD vs all other groups (P < .05). Quantification was carried out on scanned blots by densitometric analysis from ImageJ software (National Institutes of Health). (C) IL-1β enzyme-linked immunosorbent assay (ELISA) of supernatants after day 5–recovered MDSC-IL13 were plated in complete RPMI media overnight; GVHD vs all other groups (P < .05). Dotted line indicates limit of ELISA detection. All data are representative of 2 independent experiments. wt, wild-type.