Platelets from IBD patients have lower HYAL2 and hyaluronidase activity than their non-IBD counterparts. Platelets collected from IBD patients and from healthy controls, as approved by the Cleveland Clinic Institutional Review Board, were washed and lysed. Total protein concentration of platelet lysates was determined by using Bradford assay. (A) Total protein (25 µg) from each sample was analyzed by immunoblotting for HYAL2. Densitometry analysis (ImageQuant TL, GE Healthcare Life Sciences, Fairfield, CT) showed that platelets from IBD patients (n = 17) displayed an average reduction of 45% in HYAL2 protein levels compared with non-IBD controls (n = 13; P = .01). (B) Platelet lysates containing 400 µg of total protein were analyzed for their hyaluronidase activity. Platelet lysates were incubated with purified HA immobilized to wells of a 96-well plate for 18 hours at 37°C (pH 3.5). Lysates and digested HA were then washed, and remaining HA on the wells was detected colorimetrically. IBD platelets demonstrated significantly lower hyaluronidase activity (n = 8) compared with their healthy counterparts (n = 8; P < .001). Data are presented as both a scatter plot showing mean and a box-and-whiskers plot showing median of 10 to 90 percentiles.