RAE-1 is persistently induced on colonic epithelial cells in GVHD-inflicted mice treated with isotype control but not anti-NKG2D antibody. Irradiated Balb/c mice were transplanted with B6-derived BM alone or with B6-derived BM cells and FACS-sorted CD8⁺ T cells. The mice were treated 3 times per week with either isotype control antibody (IgG) or anti-NKG2D antibody. Frozen sections of colonic tissue were obtained from the mice at the indicated time points. Tissues were stained with fluorochrome-labeled Pan Keratin antibody and DAPI, and with (A) fluorochrome-labeled secondary antibody only or with RAE-1/secondary antibody in (B) TCD BM–alone mice on day 7, (C) IgG-treated mice on day 7, (D) anti-NKG2D antibody-treated mice on day 7, (E) IgG-treated mice on day 14, and (F) anti-NKG2D antibody-treated mice on day 14. Panels from left to right represent images showing fluorescence with Pan Keratin alone, RAE-1 alone, and RAE-1/Pan Keratin/DAPI. Pan Keratin is shown as green, RAE-1 as red, and DAPI as blue. (G) Sections from paraffin-embedded colons obtained on days 7 and 14 post-BMT were stained with hematoxylin and eosin from mice treated with IgG or (H) anti-NKG2D antibody. One representative of 3 independent experiments is shown.