NKG2D KO CD8⁺ T cells display defective GVT responses. Irradiated Balb/c mice were transplanted with B6-derived BM alone or with B6-derived BM cells and FACS-sorted WT or NKG2D KO CD8⁺ T cells with or without luciferase-expressing A20 cells. (A) A survival graph using all mice from 2 independent experiments (n = 8 mice/group) is shown. (B) The tumor burden measured by bioluminescence is shown as compiled data from 1 representative of 2 independent experiments (n = 4 mice/group). (C) Representative bioluminescence images of tumor-bearing mice on days 3, 23, and 57 are shown. CTL represents non–tumor-bearing mice used for measuring background bioluminescence for comparison purposes.