Prolonged anti-NKG2D antibody treatment attenuates GVT responses. Irradiated Balb/c mice were transplanted with B6-derived BM alone or with B6-derived BM cells and FACS-sorted CD8⁺ T cells with or without luciferase-expressing A20 cells (A20luc). The mice were treated 3 times per week with either isotype control antibody (IgG) or anti-NKG2D antibody for 30 days. (A) A survival graph using all mice from 2 independent experiments (n = 6 mice/group) is shown. (B) The tumor burden measured by bioluminescence is shown as compiled data from 1 representative of 2 independent experiments (n = 3 mice/group). (C) Representative bioluminescence images of tumor-bearing mice on days 3, 12, and 30 are shown. CTL represents non–tumor-bearing mice used for measuring background bioluminescence for comparison purposes.