Role of miR-17-92 on donor T cell–mediated GVL and cytolytic activities. BDF1 mice were lethally irradiated and transplanted with 5 × 106/mouse TCD-BM alone or plus purified T cells from WT or KO B6 mice at 4 × 106/mouse. Recipient mice were also infused with 5 × 103 luciferase-transduced p815 cells at the day of BMT. Recipients were monitored for survival (A) and body weight change (B) until 80 days after transplant. (C) Tumor growth was monitored using bioluminescence imaging on the dates indicated. Data were pooled from 2 replicate experiments (N = 5-10/group). Using the same BMT model, we increase the dose of p815 cells to 1 or 2.5×104/mouse, respectively. Recipients were monitored for survival (D) and mortality caused by tumor relapse (E) until 80 days after transplant (N = 6/group). In a separate experiment, recipient spleens were collected 14 days post-BMT and stained for the expression of granzyme B (GrZB), CD107a, IFNγ, and TNFα on gated CD8+ donor T cells. Representative flow figures were shown on gated donor CD8 T cells (F), and the bar graph indicates absolute numbers of total CD8 T cells or CD8 T cells that express each of these molecules (G). ***P < .001.
