miR-17-92 affects T-cell response intrinsically. Lethally irradiated BALB/c mice were transplanted with 5 × 10⁶/mouse TCD-BM (Thy1.1⁺) together with 1 × 10⁶ /mouse WT (Ly5.1⁺ Thy1.2⁺) and 1 × 10⁶ /mouse miR-17-92 KO T cells (Ly5.2⁺ Thy1.2⁺) from B6 mice. Fourteen days after BMT, recipient mice were killed and spleen, liver, lung, and intestine were harvested. Mononuclear cells were isolated from these organs and subjected for flow staining. (A) The percentage of KO (Ly5.2⁺) and WT (Ly5.1⁺) CD4 or CD8 T cells on gated on H2K^b+Thy1.2⁺ donor cells were shown in the representative flow figures. (B) BrdU was injected into recipients (2 mg/mouse) 24 hours before euthanasia of the recipients. Percentage of BrdU⁺ cells in WT and KO cells T cells were displayed on gated Ly5.1⁺ or Ly5.2⁺ cells as shown in (A). (C) Based on the percentage of WT and KO T cells in (A), we calculated the ratio of KO to WT T cells in spleen as well as in GVHD target organs. The first bar indicates 1:1 mixture of WT and KO T cells before BMT. (D) Percentages of IFNγ⁺, IL-4/5⁺, and Foxp3⁺ cells among WT or KO CD4⁺ T cells are shown. Data shown are from 1 of 2 replicate experiments (N = 10/group). *P < .05, **P < .01, ***P < .001.