Effects of miR-17-92 on the differentiation of donor T cells after allo-BMT. BMT was carried out as outlined in Figure 3. Two weeks post-BMT, recipient spleens were collected and subjected to cell counting and FACS staining. (A) The expression of IFNγ (Th1 cytokine), IL-4/5 (Th2 cytokine), and Foxp3 (Treg markers) were shown on gated donor H2K^b+Ly5.1^–CD4⁺ cells as shown in the gating strategy; (B) absolute numbers of IFNγ⁺, IL-4/5⁺, or Foxp3⁺ cells among donor H2K^b+Ly5.1^–CD4⁺ cells were shown. Few cells differentiated into IL-17–producing Th17 cells and there was no difference between WT and KO T cells (data not shown). (C) The levels of cytokines in recipient serum were measured using cytokine beads assay and displayed as mean ± SD. Data shown were pooled from 2 replicated experiments (N = 10/group). **P < .01, ***P < .001.