Role of miR-17-92 on donor T-cell migration and expression of chemokine receptor and integrin. BMT was carried out as outlined in Figure 2A, in which 1 × 10⁶/mouse donor T cells were transplanted. Fourteen days post-BMT, recipient spleens and livers were collected and mononuclear cells were isolated and subjected to cell counting and FACS staining. Absolute numbers of donor H2K^b+Ly5.1^–CD4⁺ or CD8⁺ WT (gray symbols) and KO (black symbols) T cells were shown in spleen (A) or liver (B). % CXCR3⁺ cells were shown on gated H2K^b+Ly5.1^–CD4⁺ or CD8⁺ T cells in spleen (C-D). % α4β7⁺ cells were shown on gated H2K^b+Ly5.1^–CD4⁺ or CD8⁺ T cells in spleen (E-F). Data shown were pooled from 2 replicated experiments (N = 10/group). *P < .05, **P < .01, ***P < .001.