Analysis of AMC EV-PLP preparation. (A) Representative field of an EV-PLP preparation on a hematocytometer before infusion. Bar in left lower corner indicates size of the observed field. (B) Representative size distribution of particles of the total preparation as determined by forward-scatter (FS) vs side-scatter (SS) for (left) EV-PLPs, (center) donor platelets, and (right) EV-megakaryocytes. (C) Representative surface expression of human CD41 and CD42b done on the same samples as in B. Percent of cells in each of 4 quadrants are noted. The AMC-megakaryocytes total preparation was also fractionated and gave low CD41⁺ (blue, 25% of the total) and high CD41⁺ (magenta, 73% of total) size preparations.