Size distribution of platelets in recipient mice. (A) Representative flow cytometric studies from ≥5 studies 1 hour after infusion showing forward scatter distribution as an indicator of size for the recipient mice platelets (shaded gray) compared with infused human donor platelets (dotted line) to mice studied in parallel receiving infused EV-megakaryocytes (solid line) grown from AMCs (left), FLCs (center), and iPSCs (right). (B) Representative size distribution studies after infused AMC- or iPSC-derived EV-megakaryocytes as indicated ± clodronate-ladened liposome (clod) pretreatment of the recipient NSG mice as determined by forward scatter with recipient mice platelet size as an internal size standard. (C) Mean ± SEM of ≥5 studies per arm of changes after infused AMC (top) or iPSC (bottom) EV-megakaryocytes or donor platelets that are >90% of recipient mouse platelet size. In some studies, the recipient mice had been pretreated with clodronate-ladened liposomes.