Infused human donor platelets and EV-megakaryocytes from different developmental stages into NSG mice. Donor platelets (plts) and megakaryocytes grown from AMC-, FLC-, and iPSC-derived hematopoietic progenitors were infused into NSG recipient mice. ND, not determined. (A) Degree of ploidy determined for the infused megakaryocytes before infusion showing representative studies. (B) Mean ± SEM for percent of peak human platelet counts determined over the first 48 hours after infusion. Each mouse was infused with 1 to 2.2 × 106 megakaryocytes. The half-life is indicated by a gray rectangle beginning at peak platelet count. The half-life value and the number of independent studies done are indicated in each graph. N = number of independent studies done in duplicate. (C) Same as in B, but in mice that had been pretreated intravenously with clodronate-ladened liposomes.
