Effect of BCR-ABL on miR-486-5p expression. (A) CB CD34⁺ cells were transduced with MIG-R1, MIG-R1-BCR-ABL, and MIG-R1-BCR-ABL-KI retrovirus, and CD34⁺GFP⁺ cells were sorted and analyzed for BCR-ABL expression by qPCR. (B) CB CD34⁺ cells transduced with MIG-R1, MIG-R1-BCR-ABL, and MIG-R1-BCR-ABL-KI retrovirus were cultured in GEMM medium for 3 days, and the total number of GPA, CD11b, CD33 cells was determined by FACS analysis (n = 3). (C) The expression of miR-486-5p in CB CD34⁺ cells transduced with MIG-R1, MIG-R1-BCR-ABL, and MIG-R1-BCR-ABL-KI retrovirus was examined by qPCR. (D) CML CD34⁺ cells were treated with IM at a concentration of 1 µM for 24 hours, and miR-486-5p and pri-miR-486 expression was examined by qPCR. (E) CML CD34⁺ cells were treated with IM at a concentration of 1 µM for 24 hours, and annexin V⁺ cells were detected. Cumulative results represent the mean ± SEM. *P < .05, **P < .01, ***P < .001. Ctrl, control.