Effect of miR-486-5p inhibition on growth of CD34+ cells in vivo in NSG mice. (A-E) A total of 2 × 105 CB CD34+ cells were transduced with miRZip-scramble and miRZip-anti-486-5p and transplanted into sublethally irradiated NSG mice by tail vein injection. (A) The percentage of human CD45+ cells in peripheral blood. (B) The percentage of GPA+GFP+ cells in BM at 3 weeks posttransplantation. (C) The total numbers GPA+GFP+ cells in BM at 3 weeks posttransplantation. (D) The percentage of CD34-, CD11b-, CD14-, CD33-, and CD19-expressing cells within the CD45+GFP+ population in BM at 3 weeks posttransplantation. (E) The percentage of CD34-, CD11b-, CD14-, CD33-, and CD19-expressing cells within the CD45+GFP+ population in BM at 12 weeks posttransplantation. Cumulative results represent the mean ± SEM. *P < .05, ***P < .001.
