miR-486-5p overexpression enhances the growth and erythroid differentiation of CB CD34⁺ cells. (A) The EF1-miR-486 expression cassette was cloned into lentivirus vector pHIV7-EGFP. (B) miR-486-5p and miR-486-3p expression in CB CD34⁺ cells transduced with pHIV7-EF1-miR-486 and control vector was determined by qRT-PCR. (C-F) miR-486-5p and control vector–transduced cells (Ctrl) (n = 5) were cultured in GEMM medium and GPA, CD34, CD33, CD11b, and CD14 expression analyzed by flow cytometry. The percentage of cells expressing these marker at day 3 (C) and day 6 (D) and the total number of cells expressing these markers at day 3 (E) and day 6 (F) are shown. Cumulative results represent the mean ± SEM. *P < .05, **P < .01, ***P < .001.