Comparison of survival and characterization of lymphoma in T-cell–specific PTEN-null and PTEN–phosphatase-dead mice. (A) Survival of PTEN-ΔT (ΔT) and PTEN^C124S/ΔT (CS/−) mice. (B) Bone marrow chimeras were prepared by transplanting bone marrow from PTEN-ΔT, PTEN^C124S/ΔT, or PTEN^C124S/+ (CS/+) mice into lethally irradiated WT congenic recipients. Survival of chimeric mice is measured in weeks after reconstitution. (C) Immunophenotyping of lymphoma cells from thymi of 10- to 16-week-old WT, PTEN-ΔT (ΔT), and PTEN^C124S/ΔT (CS/−) mice, representative of >10 mice. Cells were stained with antibodies to CD4, CD8, CD5, CD24, and TCRβ and were analyzed by flow cytometry. (D) Immunophenotyping of lymphoma cells from lymph nodes of 10- to 16-week-old WT, PTEN-ΔT, and PTEN^C124S/ΔT mice, representative of >10 mice. Cells were stained with antibodies to CD4, CD8, CD24, and TCRβ and were analyzed by flow cytometry. The bottom panel is gated on TCRβ⁺ cells. (E) Detection of t(14;15) by SKY analysis in metaphases from lymph nodes in PTEN^C124S/ΔT mice with lymphoma (>10-weeks-old). Note that distinct colors within a chromosome are indicative of a translocation. (F) Representative single chromosome painting specific for mouse chr14 (red) and chr15 (green) of lymphomas from PTEN^C124S/ΔT mice. Quantitation of chromosome painting is shown in supplemental Table 1 (see supplemental Data available on the Blood Web site).