Differences in gene expression between host- and donor-derived AMs. (A) Heatmap illustrating expression level profiles for gene with a significant (limma, false discovery rate = 0.05) difference for host vs donor-derived AM or CD45.1 vs CD45.2-derived AM) and at least a twofold change difference between host- and donor-derived AMs in both set 1 (host.1 vs donor.2) and set 2 (host.2 vs donor.1) (left). Probe identifiers are shown in parentheses after gene symbols annotating each row. Row-scaled expression levels shown using red-blue scale, fold-change shown using green-purple scale. Right, four-way scatter plot comparing fold change in set 1 and fold change in set 2. Genes highlighted in orange, blue, red, or green represent the 4 possible extreme groups host > donor, host < donor, CD45.1 > CD45.2, and CD45.1 < CD45.2, respectively, whose data were shown in the heatmap (left). Gray dashed lines depict a twofold change difference. (B) Scatter plot of relative expression of messenger RNA (mRNA) for genes represented on heat map. (C) Confirmation of Marco, Sema3e, Wfdc10, Wwtr1, Pldb1, and Ifi205 microarray gene expression by quantitative PCR. Data are representative of 3 independent experiments.