Transcriptional profiles of host- and donor-derived AMs. For microarray analysis, AMs of host and donor origin were fluorescence-activated cell sorted from whole lung digests from lead shielded BM reconstituted mice 8-weeks post-CLL delivery. (A) Pearson correlation plots comparing host- and donor-derived AMs from either CD45.1 (host.1 and donor.1) or CD45.2 (host.2 and donor.2). Data demonstrates experimental sets containing 4 replicates (r) of each sample type. An endothelial cell line (Bend3) was used as a control for correlation comparison (Endo). (B) Messenger RNA (mRNA) microarray expression for surface molecules on host-derived AMs (red, CD45.2 host; orange, CD45.1 host) and donor-derived AMs (blue, CD45.1 donor; light blue, CD45.2 donor) (left). Protein expression by flow cytometry for Siglec F, MertK, and F4/80 on host- and donor-derived AMs (right). Protein data represents 8 independent experiments. (C) The mRNA microarray expression levels of lung specific transcription factors. Statistically significant differences (Student t test; P < .05) between host- and donor-derived AMs are highlighted in red box. Alv Macs, alveolar macrophages.