Unidirectional generation from HTLV-1–infected T_SCM cells to T_CM and T_EM cells with rapid proliferation capacity. Data from culture of sorted T_SCM, T_CM, and T_EM cells with 25 ng/mL recombinant human IL-7 and CFSE dilution for 2 weeks are presented. (A) FACS analyses of sorted samples in each case before in vitro culture. (B) FACS plots show sorted populations from a HI, HTLV-1 carriers, and ATL patients alter their phenotype. (C) The proportions of resulting phenotypic T_SCM, T_CM, and T_EM cells shown in (B) are summarized. (D) The correlations of phenotypic alterations based on CD45RA level with proliferation ability are presented. The proliferation ability of each sorted population is assessed by the intensity of CFSE.