No infection with HTLV-1 in phenotypically sorted hematopoietic stem and progenitor cells. (A) Representative data of sorting lineage^–CD34⁺ cells in BMMCs from an ATL patient are shown. Lineage markers include CD3, CD4, CD8, CD11b, CD14, CD19, CD20, CD56, and CD235. (B) Data of PCR targeting pX as a proviral region and RNase P as an internal control are shown. The same amount of genomic DNA purified from PBMCs (0.6 ng) and lineage^–CD34⁺ cells (6 ng) sorted from BMMCs of each ATL patient are used as a template. Numbers of patients (Pt) are correspondent to those in supplemental Table 1. Positive controls indicate a plasmid DNA-cloning HTLV-1 provirus or genomic DNA of PBMCs from a HI. The left lane shows the size marker.