Effect of MYXV on the expression of IFN-γ, IL-4, and IL-10 by T cells allostimulated via MLR. To determine whether MYXV infection affects the expression of IL-2 and the IL-2 α chain receptor (IL-2Rα, aka CD25) in the setting of allostimulation via MLR, PBMCs and CB cells, from healthy donors were used to isolate MNCs. A total of 1 × 105 mock-treated (ie, no virus) responder cells or MYXV-treated responder cells were mixed with 5 × 105 irradiated stimulator cells. Cocultures were incubated for 72 hours or 6 days at 37°C, 5% CO2. At the indicated time points, supernatants were collected to carry out ELISA and multiplex assays. (A-B) ELISAs of IL-2 and IL-2Rα of PBMCs. (C-D) ELISAs of IL-2 and IL-2Rα of CB. (E-F) Multiplex assays were used to quantify the levels of secretion of IFN-γ, IL-4, and IL10 from PBMCs and CB cells, respectively.
