Akt/β-catenin/survivin signaling pathway is upregulated by Runx2 in MM cells. (A) Expression of Runx2, phosphorylated Akt, total Akt, active β-catenin, survivin, and β-actin in Runx2 k/in 5TGM1 cells and control cells analyzed by western blot. (B) Expression of Runx2, phosphorylated Akt, total Akt, active β-catenin, survivin, and β-actin in Runx2 k/d 5TGM1 cells (Runx2 shRNA #90 and #91) compared with NT control cells. (C) Expression of Runx2, phosphorylated Akt, total Akt, active β-catenin, survivin, and β-actin in NT control and Runx2 k/d MM.1R cells analyzed by western blot. (D) Runx2 k/in cells were cultured with the Akt inhibitor MK2206 (2.5 μM) or without (DMSO control) and analyzed for phosphorylated Akt, total Akt, active β-catenin, survivin, and β-actin by western blot. (E) Invasion assays were performed with Runx2 k/in cells treated with DMSO (Control) (□) or MK2206 (2.5 μM) (▪) for 24 or 48 hours. Each sample was analyzed in triplicate and each assay was performed 3 times. Error bars represent mean ± SEM of each group. Significant differences between groups are indicated by P value.