Ott1 binds c-Mpl RNA and chromatin. (A) RNA immunoprecipitation (RIP) from HA-Ott1 or GFP control–infected NIH3T3 cells using anti-HA antibody. RNA immunoprecipitates and input lysate RNA were reverse transcription-PCR (RT-PCR)–amplified for exon 5/6 of c-Mpl and Gapdh controls. Agarose gel of RT-PCR products from RIP and input (left panel). Ratio of output:input of qPCR of RIP and input lysate cDNA (n = 3; error bars ± SD). (B) Chromatin immunoprecipitation (ChIP) from HA-Ott1 (gray columns) or GFP control (white)-infected NIH3T3 cells using anti-HA antibody. Ratio of output:input from HA-Ott1–expressing cells compared with GFP-expressing controls using qPCR, with primers directed toward the indicated regions (n = 3; error bars, SD; dotted line, Mpl-TR splice; solid line, Mpl-FL splice).