miR-486 is important for the ML-DS erythroid phenotype. (A) GYPA (glycophorin A and CD235) expression in diagnostic BM of ML-DS patients (n = 25) compared with non–DS-AMKL (n = 38), published microarray data.3 (B) Pearson correlation test between the expression level of GYPA and miR-486-5p determined by qRT-PCR in diagnostic BM of 36 ML-DS patients. **Correlation was statistically significant, P < .01 (ANOVA). (C) Expression of miR-486-5p in CMK and CMY ML-DS cells stably expressing shRNA anti–miR-486-5p (miR-486-5p-KD) or SCR. (D) GYPA and CD61 expression in CMK ML-DS cells stably expressing shRNA anti–miR-486-5p or SCR determined by flow cytometry. (E) Increase of cells with pure megakaryocytic phenotype (GYPA− and CD61+) in CMK and CMY ML-DS cells stably expressing shRNA anti–miR-486-5p in comparison with SCR. *P < .01 (2-tailed Student t test). SCR, scrambled.
