TLR ligands increase PLCγ2 phosphorylation in CLL subset #8 CLL but not in subsets #1 or #2. (A) Densitometric analysis of p-PLCγ2/PLCγ2 normalized to the unstimulated control, in CLL samples after stimulation with TLR ligands: PAM3CSK4, CpG ODN 2006, and MALP-2. The graphs represent the mean values in subsets #8, #1, and #2 CLL cases (5, 4, and 2 cases, respectively).*P < .05. (B) Representative western blot of a subset #8 case displaying increased PLCγ2 phosphorylation after stimulation with anti-IgG Ab and TLR ligands PAM3CSK4, CpG ODN 2006, and MALP-2, contrasting a nonresponding subset #1 case.