Binding patterns of CLL mAbs assigned to stereotyped subsets #1, #2, and #8 to various autoantigens. Reactivity of CLL recombinant mAbs with common extractable nuclear antigens, including Sm, Jo-1, U1-RNP, and SS-A/Ro (A), goat IgG (B), and dsDNA (C). CLL mAbs were used as primary Ab for the ELISA. Samples were grouped on the basis of BCR stereotypy. The bars represent the mean OD value measured for CLL mAbs assigned to each of the stereotyped subsets #1, #2, or #8. The mAbs tested and the mean OD values of 3 replicates per sample are listed in supplemental Table 5. Rheumatoid factor activity was tested by commercial ELISA kit (C); positive and cutoff controls were provided by the kit. For anti-dsDNA activity (B), serum of a patient with systemic lupus erythematosus was used as positive control in a 1:200 dilution. *P < .05; **P < .005; ***P < .001. Cut-off, cutoff control; Pos cntr, positive control.