Heterozygous loss of Ebf1 results in lower levels of Rad51 in B-cell progenitors. (A) Quantitative RT-PCR data from in vitro-cultured Wt or Ebf1^+/− pro-B cells. The data are normalized to the expression of HPRT in triplicate PCR reactions and represent 3 independent experiments. The Student t test was performed to check the statistical significance. **P < .01 and *P < .05. (B) Immunofluorescence staining of γH2AX (rabbit) and mouse Rad51 in in vitro-cultured Wt or Ebf1^+/− pro-B cells followed by the specific anti-Alexa Fluor 594 (γH2AX) and anti-Alex Fluor 488 secondary antibody (Rad51). DAPI was used to stain the nucleus. The panel adjacent to each IF image shows a quantification of the signal intensity collected from 3 experiments, and quantification is represented as fold integrated density with statistical significant value: *P < .05 for γH2AX and ***P < .001 for Rad51. (C) Immunohistochemical staining of Rad51 in in vitro-cultured Wt or Ebf1^+/− pro-B cells 16 hours after UV exposure. DAPI was used to stain the nucleus. The data were collected from 3 experiments, and foci formation was counted from 3 cells from 3 different experiments. The error bars in the diagrams indicate mean ± SD, and statistical analysis was performed using the Student t test, with P < .001.