CLEC-2 downregulation occurs through Syk-independent internalization. (A) Wt and Syk^−/− platelets were in vitro incubated with 20 µg/mL INU1-F488 for 15 minutes. Thereafter, platelets were stained and imaged as described in Figure 3F. Scale bars represent 1 µm. (B) Wt and Syk^−/− platelets were in vitro incubated with 20 µg/mL INU1-F488 for 15 minutes followed by 2-minute incubation with Thr (0.1 U/mL). Subsequently, platelets were stained and imaged as described in Figure 3F. Results are representative of 3 individual experiments.