DGKE knockdown induces activation of p38- and p44/42-MAP kinases in ECs. (A) HUVECs were transfected with control or DGKE siRNAs (20 nM) and after 48 hours, a phosphokinase protein array was performed on cell lysates to analyze the relative expression of 46 individual kinases or some of their target proteins. The bar graph illustrates the fold change in pixel density for each protein of the array in DGKE siRNA–transfected cells vs control cells. (B) The expression of phospho-p44/42-MAPK (T202/Y204), total p44/42-MAPK, phospho-p38-MAPK (T180/Y182), and total p38-MAPK, as well as (C) phospho-PKC (S660), total PKC, and β-actin was examined in control or DGKE siRNA–transfected HUVECs using western blot analyses. Representative results of 3 independent experiments are shown.