HGAL colocalizes in cellular membrane with lipid raft proteins. Confocal and differential interference contrast images of HGAL-GFP-expressing MCF7 cells that were cross-linked by cholera toxin B. Maximum-intensity images were generated from a series of 30 stack images acquired at a thickness of 0.5 μm. (A) HGAL channel. (B) Cholera toxin B channel. (C) Cholera toxin B overlaid on HGAL. (D) Differential interference contrast image. Results are representative of 2 independent experiments. Bar = 10 μm.