Figure 3
Results of STAT6 luciferase assays in HEK293T cells across a panel of luciferase response plasmids. (A) Results for an eotaxin-3 luciferase (Luc) plasmid and two synthetic STAT6 luciferase response plasmids described by Ritz et al,35 each carrying 3 STAT6 sites (supplemental Methods); N3: TTCNNNGAA; N4: TTCNNNNGAA. Numbers indicate fold-activation over the unstimulated STAT6 wt signal. (B-C) Results for luciferase assays using naturally occurring genomic DNA stretches carrying STAT6 site(s) derived from CISH and FCER2 and corresponding immunoblot results. (D) Results for luciferase assays for 5 naturally occurring genomic DNA stretches carrying single STAT6 sites in AGAP9, CCL17, FCER2, and two NFIL3-derived sites.
