Upregulation of IL-18R and IFN-γ production in response to alloantigen is reduced in st2^−/− T cells. (A-B) Gene expression was quantified in WT and st2^−/− T cells on the RNA level by microarray analysis. WT or st2^−/− T cells were exposed to allogeneic irradiated DC for 48 hours. The tile display for the most significantly regulated genes expressed by RMA signal values of 4 individual samples in each group shown at the RNA level. (C) The values of individual mice for serum IFN-γ is shown on day 8 after allo-HCT. The experiment was performed twice and the data were pooled. (D) WT and st2^−/− CD4⁺/CD8⁺ T cells stimulated with allo–BM-DCs (2:1 ratio). ELISA for IFN-γ was performed after 24 hours of exposure. The experiment was performed twice with similar results.