miR-10a suppresses monocyte/macrophage activation. (A) MiR-10a overexpression in THP-1 monocytic cells suppresses the induction of proinflammatory genes in response to LPS stimulation, as assessed by qRT-PCR. n = 6. (B) MiR-10a overexpression in ECs suppresses the induction of proinflammatory genes in cocultured THP-1 cells stimulated with LPS. Values are expressed relative to cocultured cells transfected with control mimic, and the suppressive effective of miR-10a overexpression is therefore in addition to the effect of EC coculture on monocytic cell activation. n = 6. (C) Overexpression of miR-10a in mouse bone marrow–derived macrophages (BMDMs) suppresses the expression of proinflammatory genes. Shown are unstimulated BMDMs (left) or BMDMs treated with LPS/interferon-γ (right). n = 6 and n = 5, respectively. (D) Inhibiting miR-10a in THP-1 monocytic cells negates the EC-EV–dependent downregulation of proinflammatory genes and affects the expression of the immunomodulatory marker MRC1 in LPS-stimulated cells. Data are expressed relative to cells transfected with control inhibitor with no EC-EVs. n = 6.