RUNX1T1 methylation, expression, and 2HG level correlation. (A) Methylation of RUNX1T1 is enriched in the intragenic region in TF-1 R140Q cells (clone 11 and clone 17) compared with TF-1 pLVX (n = 12). (B) Gene map: black and white bar (top), chromosomal position; light green bar (bottom), gene structure, with exons as maroon vertical bars. (C) Methylation in the promoter region in TF-1 R140Q cells (clone 11 and clone 17) compared with TF-1 pLVX (n = 3; gray bar, chromosomal position). (D) Increase in RUNX1T1 mRNA expression is observed in TF-1 R140Q cells, which can be reversed (as measured by real-time PCR) after 7 days of treatment with AGI-6780. The decrease in expression also correlates with a dose-dependent decrease in the production of 2HG on compound treatment. Error bars = standard deviation.