Tail clip injury model showing comparable efficacy as BDD-FVIII with half the mass dose for D519V/E665V. HemA mice were dosed with buffer (excipient); 0.6, 2, or 6 µg/kg D519V/E665V or 4 µg/kg BDD-FVIII. The BDD-FVIII dose represents the ED₅₀ for this molecule in these studies. Statistical significance (*P < .05, 1-way ANOVA with Dunnett’s multiple comparison test) was achieved with 2 µg/kg D519V/E665V vs excipient, whereas 4 µg/kg BDD-FVIII was required to achieve the same level of statistical difference vs excipient. †0.6 U/kg = 2 µg/kg D519V/E665V = 4 µg/kg BDD-FVIII. ANOVA, analysis of variance.